Pyrroloquinoline quinone-dependent glucose dehydrogenase bioelectrodes based on one-step electrochemical entrapment over single-wall carbon nanotubes

Development of effective direct electron transfer is considered an interesting platform to obtain high performance bioelectrodes. Therefore, designing scalable and cost-effective immobilization routes that promotes correct electrical contacting between the electrode material redox enzyme still required. As we present here, electrochemical entrapment pyrroloquinoline quinone-dependent glucose dehydrogenase (PQQ-GDH) on single-wall carbon nanotube (SWCNT)-modified electrodes was carried out in a single step during electrooxidation para -aminophenyl phosphonic acid (4-APPA) active The adequate interaction SWCNTs can be achieved by making use phosphorus groups introduced co-deposition films, improving electrocatalytic activity towards oxidation. Two different procedures were investigated for fabrication, namely reconstituted holoenzyme apoenzyme (apo-GDH) followed subsequent situ reconstitution with cofactor PQQ. In both cases, PQQ-GDH preserves its Moreover, comparison conventional drop-casting method, important enhancement sensitivity obtained oxidation (981.7 ± 3.5 nA mM −1 ) using substantially lower amounts (PQQ). presence 4-APPA provides simple method fabrication enzymatic • Electrochemical nanotubes has been performed. 4-APPA.